(1) Background: Frequent fire, climate variability, and human activities collectively influence savanna ecosystems. The relative role of these three factors likely varies on interannual, decadal, and centennial timescales. Here, we tested if Euro-American activities uncoupled drought and fire frequencies relative to previous centuries in a temperate savanna site. (2) Methods: We combined records of fire frequency from tree ring fire scars and sediment charcoal abundance, and a record of fuel type based on charcoal particle morphometry to reconstruct centennial scale shifts in fire frequency and fuel sources in a savanna ecosystem. We also tested the climate influence on fire occurrence with an independently derived tree-ring reconstruction of drought. We contextualized these data with historical records of human activity. (3) Results: Tree fire scars revealed eight fire events from 1822–1924 CE, followed by localized suppression. Charcoal signals highlight 13 fire episodes from 1696–2001. Fire–climate coupling was not clearly evident both before and after Euro American settlement The dominant fuel source shifted from herbaceous to woody fuel during the early-mid 20th century. (4) Conclusions: Euro-American settlement and landscape fragmentation disrupted the pre-settlement fire regime (fire frequency and fuel sources). Our results highlight the potential for improved insight by synthesizing interpretation of multiple paleofire proxies, especially in fire regimes with mixed fuel sources.